Citation

Morley M, Gee DJ. J. Forensic Sci. 1982; 27(4): 837-843.

Copyright

(Copyright © 1982, American Society for Testing and Materials, Publisher John Wiley and Sons)

DOI

unavailable

PMID

6294214

Abstract

The use of the indirect immunofluorescence technique as a method for demonstrating the histological localization of tetrahydrocannabinol (delta-THC) has been examined. The experimental protocol was designed in order that optimal staining conditions with respect to temperature, the length of time of incubations and washes, and the dilution of the antisera should be defined. No marked differences were detected between frozen sections of liver from normal and delta-THC-injected mice. Results from radiotracer experiments using human liver suggest that the success of the method is dependent upon the solubility characteristics of the antigen-antibody complex.

Language: en