Citation

da Silva NC, Honorato RS, Pimentel MF, Garrigues S, Cervera ML, de la Guardia M. J. Forensic Sci. 2015; 60(5): 1199-1205.

Affiliation

Department of Analytical Chemistry, University of Valencia, Valencia, Spain.

Copyright

(Copyright © 2015, American Society for Testing and Materials, Publisher John Wiley and Sons)

DOI

10.1111/1556-4029.12884

PMID

26260573

Abstract

There is an increasing demand for herbal medicines in weight loss treatment. Some synthetic chemicals, such as sibutramine (SB), have been detected as adulterants in herbal formulations. In this study, two strategies using near infrared (NIR) spectroscopy have been developed to evaluate potential adulteration of herbal medicines with SB: a qualitative screening approach and a quantitative methodology based on multivariate calibration. Samples were composed by products commercialized as herbal medicines, as well as by laboratory adulterated samples. Spectra were obtained in the range of 14,000-4000 per cm. Using PLS-DA, a correct classification of 100% was achieved for the external validation set. In the quantitative approach, the root mean squares error of prediction (RMSEP), for both PLS and MLR models, was 0.2%w/w. The results prove the potential of NIR spectroscopy and multivariate calibration in quantifying sibutramine in adulterated herbal medicines samples.

Language: en