Citation

Nomura C, Masayama A, Yamaguchi M, Sakuma D, Kajimura K. Shokuhin Eiseigaku Zasshi 2017; 58(3): 132-142.

Affiliation

Osaka Prefectural Institute of Public Health.

Copyright

(Copyright © 2017, Food Hygienic Society of Japan)

DOI

10.3358/shokueishi.58.132

PMID

28690303

Abstract

In this study, species-specific identification of five toxic mushrooms, Chlorophyllum molybdites, Gymnopilus junonius, Hypholoma fasciculare, Pleurocybella porrigens, and Tricholoma ustale, which have been involved in food-poisoning incidents in Japan, was investigated. Specific primer pairs targeting internal transcribed spacer (ITS) regions were designed for PCR detection. The specific amplicons were obtained from fresh, cooked, and simulated gastric fluid (SGF)-treated samples. No amplicons were detected from other mushrooms with similar morphology. Our method using one-step extraction of mushrooms allows rapid detection within 2.5 hr. It could be utilized for rapid identification or screening of toxic mushrooms.

Language: en

Keywords

Chlorophyllum molybdites; DNA barcoding; Gymnopilus junonius; Hypholoma fasciculare; Pleurocybella porrigens; Tricholoma ustale; food-poisoning incidents; mushroom; simulated gastric fluid