@article{ref1,
title="The N251K functional polymorphism in the alpha(2A)-adrenoceptor gene is not associated with depression: a study in suicide completers",
journal="Psychopharmacology",
year="2006",
author="Martín-Guerrero, Idoia and Callado, Luis F. and Saitua, Koldo and Rivero, Guadalupe and García-Orad, Africa and Meana, J. J.",
volume="184",
number="1",
pages="82-86",
abstract="RATIONALE: alpha(2A)-Adrenoceptor up-regulation and supersensitivity have been described in the postmortem brains of depressed suicide victims and in the platelets of depressed subjects. The C to G transversion at nucleotide 753 (Asn to Lys change at amino acid 251 or N251K) is a low-frequency polymorphism of the alpha(2A)-adrenoceptor gene that results in a gain-of-function phenotype. A previous study has suggested an association between completed suicide and this polymorphism. OBJECTIVES: The single functional polymorphism N251K was tested in a large sample (n=214) of completed suicides, controlling for the antemortem psychiatric diagnosis, and matched controls (n=176). METHODS: Postmortem brain DNA was extracted and the alpha(2A)-adrenoceptor gene fragment was amplified by polymerase chain reaction, followed by a StyI restriction endonuclease digestion. Amplified products were sequenced to confirm the presence of the alpha(2A)-adrenoceptor gene fragment where the polymorphism is located. RESULTS: The N251K polymorphism was absent in both suicide victim and control groups. No association between the polymorphism and suicide or depression was established. CONCLUSIONS: The N251K polymorphism does not represent a genetic factor to explain the alpha(2A)-adrenoceptor hyperactivity in the brains of depressed suicide victims. Association between suicide and this polymorphism was not replicated.<p /> <p>Language: en</p>",
language="en",
issn="0033-3158",
doi="10.1007/s00213-005-0266-2",
url="http://dx.doi.org/10.1007/s00213-005-0266-2"
}