@article{ref1,
title="M-type K+ current inhibition by a toxin fron the scorpion Buthus eupeus",
journal="FEBS letters",
year="1996",
author="Filippov, A. K. and Kozlov, S. A. and Pluzhnikov, K. A. and Grishin, Eugene V. and Brown, D. A.",
volume="384",
number="3",
pages="277-280",
abstract="A number of invertebrate venoms have been tested for effects on M-type K+ currents (IK(M)) in differentiated mouse neuroblastoma X rat glioma NG108-15 cells. Among the venoms tested, Buthus eupeus scorpion venom reversibly inhibited IK(M) by approximately 44% at 50 microgram/ml. Inhibition was not due to activation of bradykinin or nucleotide (pyrimidine) receptors. On venom fractionation, a polypeptide of 4 kDa was purified that inhibited IK(M) by approximately 45% with an IC50 of approximately 33 nM. Neither the crude venom nor the purified polypeptide affected the Ca2+ current or the delayed rectifier K+ current. While the crude venom prolonged the Na+ current, the polypeptide did not. Thus, the 4 kDa Buthus eupeus polypeptide appears to be a selective inhibitor of IK(M) in NG108-15 cells.<p /><p>Language: en</p>",
language="en",
issn="0014-5793",
doi="",
url="http://dx.doi.org/"
}