@article{ref1,
title="Cytotoxicity of extracts from the mushroom Paxillus involutus",
journal="Toxicon: Journal of the International Society on Toxinology",
year="1996",
author="Habtemariam, S.",
volume="34",
number="6",
pages="711-713",
abstract="The cytotoxicity of extracts of freshly collected Paxillus involutus against murine (L929 and RAW 264.7) and human (HeLa and EAhy926) derived cell lines was investigated. The water extract (15.6-1000 micrograms/ml) did not affect the viability of cells, while the butanol extract reduced cell viability by 30-45% at the highest concentration tested (1 mg/ml). The ethyl acetate extract was cytotoxic in a concentration-dependent manner, with IC50 values between 125 and 250 micrograms/ml. The cytotoxic principle appears to be heat and acid stable, as neither incubation at 37 degrees C for 4 hr nor overnight treatment at pH 0.5 altered the cytotoxicity of the ethyl acetate extract. This non-polar, acid- and thermo-stable cytotoxic principle may play a role in the haemolysis and other clinical manifestations that occur during poisoning by P. involutus.<p /><p>Language: en</p>",
language="en",
issn="0041-0101",
doi="",
url="http://dx.doi.org/"
}