@article{ref1,
title="Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1",
journal="Toxicon: Journal of the International Society on Toxinology",
year="2010",
author="Dias-Lopes, Camila and Felicori, Liza and Guimarães, G and Gomes, Eneas R.M. and Roman-Campos, Danilo and Duarte, Hugo and Damasceno, Denis and Martins, Marilia and Kalapothakis, Evanguedes and Almeida, Alvair P. and Granier, Claude and Cruz, Jader S. and Guatimosim, Silvia and Chávez-Olórtegui, C.",
volume="56",
number="8",
pages="1426-1435",
abstract="Loxosceles spider bites cause many human injuries worldwide. Injections in mice of whole Loxosceles (L.) intermedia venom or a recombinant toxin (rLiD1) produce systemic symptoms similar to those detected in envenomed humans. This animal model was used to characterize the effects of L. intermedia venom in cardiac tissues. L. intermedia antigens were detected by ELISA in kidney, heart, lung and liver of experimentally envenomed mice. In addition, rLiD1 binding to cardiomyocytes was demonstrated by immunofluorescence and confocal microscopy. Furthermore, isolated perfused heart preparations and ventricular cardiomyocytes from envenomed mice showed heart function impairment, and a significant increase of I(Ca,L) density and intracellular Ca(2+) transients, respectively. Thus, L. intermedia spider venom, as shown through the use of the recombinant toxin rLiD1, causes cardiotoxic effects and a protein from the sphingomyelinase family plays a key role in heart dysfunction. Thus, L. intermedia spider venom and the Loxtox rLiD1 play a key role in heart dysfunction.<p /> <p>Language: en</p>",
language="en",
issn="0041-0101",
doi="10.1016/j.toxicon.2010.08.008",
url="http://dx.doi.org/10.1016/j.toxicon.2010.08.008"
}