@article{ref1,
title="Neurotoxic activity of Gln49 phospholipase A(2) from Gloydius ussuriensis snake venom",
journal="Journal of applied toxicology",
year="2007",
author="Chang, Yuefang and Li, Yun and Bao, Yanjun and An, Lijia",
volume="27",
number="5",
pages="447-452",
abstract="A novel neurotoxic protein phospholipase A(2) (PLA(2)), molecular weight 13 881.83 Da, has been isolated from snake venom of Gloydius ussuriensis, named as Gln49-PLA(2), which shows weak lethal toxic, myotoxic and apparent anticoagulant activity, but lacks phospholipase activity. The Gln49-PLA(2) obviously induced an increase of the pain threshold in intoxicated 615 mice compared with the control group, suggesting it is a neurotoxin. Hot-plate tests also showed that its analgesic activity was dose-dependent, and naloxone antagonized the analgesic effect, implying the mechanism of action of Gln49-sPLA(2) is correlated with opioid receptors. Electrophysiology studies revealed decreases in the action potential and the nerve conduction velocity in isolated hoptoad (Bufo bufo gargarizans Cantor) sciatic nerve, indicating Gln49-PLA(2) most probably had effects on ion channels.<p /><p>Language: en</p>",
language="en",
issn="0260-437X",
doi="10.1002/jat.1222",
url="http://dx.doi.org/10.1002/jat.1222"
}