@article{ref1,
title="Protective effects of melatonin on ischemia-reperfusion injury of skeletal muscle",
journal="Eklem hastalıkları ve cerrahisi",
year="2010",
author="Erdem, Mehmet and Bostan, Bora and Güneş, Taner and Ozkan, Fatih and Sen, Cengiz and Ozyurt, Hüseyin and Köseoğlu, Reşit Doğan and Erdoğan, Hasan",
volume="21",
number="3",
pages="166-171",
abstract="Objectives: In this study, we investigated the antioxidant protective effects of melatonin on skeletal muscles of Wistar albino-type rats with acute ischemia/reperfusion (I/R) injury. Materials and methods: Twenty-eight Wistar albino-type male rats weighing between 334 to 422 g were included in this experimental study. The rats were randomly allocated into three groups including sham, I/R and I/R + melatonin groups, respectively. Limb ischemia was achieved by clamping femoral arteries. After a two-hour ischemia period followed by 1.5-hour reperfusion, muscle samples were collected for biochemical analysis and histopathological examination. Results: Muscle tissues of I/R groups revealed significantly higher antioxidant enzyme (superoxide dismutase, glutathione peroxidase, catalase) activities, and increased levels of malondialdehyde, nitric oxide, and protein carbonyl content compared to the control group (p<0.001). Levels of these parameters in muscle revealed significant reductions in the I/R + melatonin group compared to the I/R group (p<0.001). Histopathological examination of ischemic muscles in the I/R group showed significant degeneration and inflammation compared to the control group whereas melatonin administered ischemic muscles showed significant reduction in degeneration and inflammation compared to the I/R group (p<0.001). Conclusion: In the present skeletal muscle acute I/R injury model, protective effects of melatonin against reperfusion injury have been revealed. We suggest that the protective effect of melatonin against I/R damage in cases of extremity injuries with acute vascular compromise, extremity surgery with prolonged tourniquet time and acute compartment syndrome should be investigated with clinical trials.<p /> <p>Language: tr</p>",
language="tr",
issn="1305-8282",
doi="",
url="http://dx.doi.org/"
}