@article{ref1,
title="First approach of a methodological set‐up for selenomethionine chiral speciation in breast and formula milk using high‐performance liquid chromatography coupled to atomic fluorescence spectroscopy",
journal="Applied organometallic chemistry",
year="2007",
author="Gómez‐Ariza, J. L. and Bernal‐Daza, V. and Villegas‐Portero, M. J.",
volume="21",
number="6",
pages="434-440",
abstract="The chiral speciation of selenomethionine in breast and formula milk based on species separation by high-performance liquid chromatography followed by online microwave-assisted digestion and detection with hydride generation atomic fluorescence spectrometry (HPLC-MAD-HG-AFS) requires severe sample manipulation to avoid matrix influence. Sample clean-up for fat and protein elimination using centrifugation and ultrafiltration was optimized, and selenomethionine preconcentration based on cation exchange solid-phase extraction was studied and optimized. The resulting procedure is suitable for chiral selenium speciation in infant milk with detection limits of 3.1 and 3.5 ng ml−1 as Se for L-selenomethionine and D-selenomethionine, respectively. The time necessary for the analysis, about 90 min, including sample clean-up, analyte preconcentration and chromatographic separation, makes the approach suitable for routine analysis. Copyright © 2007 John Wiley & Sons, Ltd.<p />",
language="",
issn="0268-2605",
doi="10.1002/aoc.1239",
url="http://dx.doi.org/10.1002/aoc.1239"
}