@article{ref1,
title="Detection of ricin contamination in ground beef by electrochemiluminescence immunosorbent assay",
journal="Toxins (Basel)",
year="2011",
author="Brandon, David L.",
volume="3",
number="4",
pages="398-408",
abstract="Ricin is a highly toxic protein present in the seeds of Ricinus communis (castor), grown principally as a source of high quality industrial lubricant and as an ornamental. Because ricin has been used for intentional poisoning in the past and could be used to contaminate food, there is a need for analytical methodology to detect ricin in food matrices. A monoclonal antibody-based method was developed for detecting and quantifying ricin in ground beef, a complex, fatty matrix. The limit of detection was 0.5 ng/g for the electrochemiluminescence (ECL) method and 1.5 ng/g for enzyme-linked immunosorbent assay (ELISA). The detection of nanogram per gram quantities of ricin spiked into retail samples of ground beef provides approximately 10,000-fold greater sensitivity than required to detect a toxic dose of ricin (>1 mg) in a 100 g sample.<p /> <p>Language: en</p>",
language="en",
issn="2072-6651",
doi="10.3390/toxins3040398",
url="http://dx.doi.org/10.3390/toxins3040398"
}