@article{ref1,
title="Detection of exogenous intake of natural corticosteroids by gas chromatography/combustion/isotope ratio mass spectrometry: application to misuse in sport",
journal="Rapid communications in mass spectrometry",
year="2000",
author="Bourgogne, E. and Herrou, V. and Mathurin, J. C. and Becchi, M. and de Ceaurriz, J.",
volume="14",
number="24",
pages="2343-2347",
abstract="A detailed procedure for the analysis of exogenous hydrocortisone and cortisone in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is proposed. As urinary levels of hydrocortisone are rather low for GC/C/IRMS analysis, the focus is on the main corticosteroid metabolites, tetrahydrocortisone (THE) and tetrahydrocortisol (THF). Following different solid phase extraction purifications, THE and THF are oxidized to 5beta-androstanetrione before analysis by GC/C/IRMS. Significant differences in delta(13)C per thousand values of synthetic natural corticosteroids and endogenous human corticosteroids have been observed. Therefore, a positive criterion, to detect exogenous administration of synthetic corticosteroids in anti-doping control, is proposed.<p /><p>Language: en</p>",
language="en",
issn="0951-4198",
doi="10.1002/1097-0231(20001230)14:24<2343::AID-RCM171>3.0.CO;2-Z",
url="http://dx.doi.org/10.1002/1097-0231(20001230)14:24<2343::AID-RCM171>3.0.CO;2-Z"
}