@article{ref1,
title="A vaccine against methamphetamine attenuates its behavioral effects in mice",
journal="Drug and alcohol dependence",
year="2013",
author="Shen, Xiaoyun Y. and Kosten, Therese A. and Lopez, Angel Y. and Kinsey, Berma M. and Kosten, Thomas R. and Orson, Frank M.",
volume="129",
number="1-2",
pages="41-48",
abstract="BACKGROUND: Vaccines have treatment potential for methamphetamine (MA) addiction. We tested whether a conjugate vaccine against MA (succinyl-methamphetamine-keyhole limpet hemocyanin carrier protein; SMA-KLH) would generate MA antibodies and alter MA-induced behaviors. METHODS: Mice were injected with SMA-KLH and received booster administrations 3 and 20 weeks later. Serum antibody titers reached peak levels by 4-6 weeks, remained at a modest level through 18 weeks, peaked again at 22 weeks after the second boost, and were still elevated at 35 weeks. At 7weeks, groups of vaccinated and non-vaccinated mice were administered one of three MA doses (1, 2 or 3mg/kg) to assess locomotor activity. RESULTS: Non-vaccinated mice showed dose-dependent effects of MA with hypolocomotion at the lowest dose and elevated activity levels at the highest dose. Both dose effects were reduced in SMA-KLH groups, particularly low dose-induced hypolocomotion at later times post MA administration. Separate groups of vaccinated and non-vaccinated mice were trained in MA place conditioning at 30 weeks with either 0 (vehicle) or 0.5mg/kg MA. Although times spent in the MA-paired side did not differ between groups on test vs. baseline sessions, SMA-KLH mice conditioned with MA showed reduced conditioned approach behaviors and decreased conditioned activity levels compared to control groups. CONCLUSION: These data suggest SMA-KLH attenuates the ability of MA to support place conditioning and reduces or delays its locomotor effects. Overall, results support SMA-KLH as a candidate MA vaccine.<p /> <p>Language: en</p>",
language="en",
issn="0376-8716",
doi="10.1016/j.drugalcdep.2012.09.007",
url="http://dx.doi.org/10.1016/j.drugalcdep.2012.09.007"
}