@article{ref1,
title="Physiological and biochemical analysis to reveal the molecular basis for black widow spiderling toxicity",
journal="Journal of biochemical and molecular toxicology",
year="2014",
author="Peng, Xiaozhen and Zhang, Yiya and Liu, Jinyan and Yu, Hai and Chen, Jia and Lei, Qian and Wang, Xianchun and Liang, Songping",
volume="28",
number="5",
pages="198-205",
abstract="The early research found that the spiderlings of black widow spider (Latrodectus tredecimguttatus) exhibited obvious toxicity to animals. The present work performed a systematical analysis of the aqueous extract of newborn black widow spiderlings. The extract was shown to contain 69.42% of proteins varying in molecular weights and isoelectric points. Abdominal injection of the extract into mice and cockroaches caused obvious poisoning symptoms as well as death, with LD50 being 5.30 mg/kg in mice and 16.74 µg/g in Periplaneta americana. Electrophysiological experiments indicated that the extract at a concentration of 10 µg/mL could completely block the neuromuscular transmission in isolated mouse nerve-hemidiaphragm preparations within 21 ± 1.5 min, and 100 µg/mL extract could inhibit a certain percentage of voltage-activated Na(+) , K(+) , and Ca(2+) channel currents in rat dorsal root ganglion neurons. These results demonstrate that the spiderlings are rich in neurotoxic components, which play important roles in the spiderling toxicity.<p /> <p>Language: en</p>",
language="en",
issn="1095-6670",
doi="10.1002/jbt.21553",
url="http://dx.doi.org/10.1002/jbt.21553"
}