@article{ref1,
title="A highly rapid and simple competitive enzyme-linked immunosorbent assay for monitoring paralytic shellfish poisoning toxins in shellfish",
journal="Food chemistry",
year="2014",
author="Kawatsu, Kentaro and Kanki, Masashi and Harada, Tetsuya and Kumeda, Yuko",
volume="162",
number="",
pages="94-98",
abstract="Using a streptavidin-coated well plate, a biotin-labelled anti-gonyautoxin 2/3 monoclonal antibody GT-13A, and a decarbamoyl saxitoxin-peroxidase conjugate, a direct competitive enzyme-linked immunosorbent assay (PSP-ELISA) was developed for monitoring paralytic shellfish poisoning (PSP) toxins in shellfish. This assay is simple to perform and can be completed in approximately 20min. The PSP-ELISA was compared to the mouse bioassay (MBA) for the detection of PSP toxins in shellfish samples (n=83) collected from the coast of Osaka Prefecture, Japan. When positive and negative results were indicated based on the regulatory limit for PSP toxins (4mouseunit(MU)/g of shellfish meat), the PSP-ELISA results showed a sensitivity of 100% (25 of 25) and a specificity of 89.7% (52 of 58 samples) compared to the MBA results. These results suggest that the PSP-ELISA could be used as a rapid and simple screening method prior to the MBA.<p /><p>Language: en</p>",
language="en",
issn="0308-8146",
doi="10.1016/j.foodchem.2014.04.038",
url="http://dx.doi.org/10.1016/j.foodchem.2014.04.038"
}