@article{ref1,
title="Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case",
journal="Croatian medical journal",
year="2014",
author="Hu, Lan and Li, Caixia and Tu, Zheng and Li, Wanshui and Yang, Fan and Zhang, Huaicai and Zeng, Xianhai and Xu, Cheng and Feng, Lei",
volume="55",
number="5",
pages="537-541",
abstract="Aim. To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. <br><br>METHODS. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler® kit. Consensus DNA profiles were generated from 5 replicate experiments. <br><br>RESULTS. Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained. <br><br>CONCLUSION. The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.<p /> <p>Language: en</p>",
language="en",
issn="0353-9504",
doi="",
url="http://dx.doi.org/"
}