@article{ref1,
title="Evaluation of three methods for forensic diatom test",
journal="Nan Fang Yi Ke Da Xue Xue Bao",
year="2015",
author="Wang, Yu-Zhong and Zhao, Jian and Li, Peng and Hu, Sun-Lin and Wang, Hui-Pin and Wang, Hui-Jun and Liu, Chao",
volume="35",
number="3",
pages="427-431",
abstract="OBJECTIVE: To compare the efficacy of three methods for forensic diatom test, namely strong acid digestion-centrifuge enrichment-light microscopy (SD-CE-LM), microwave digestion-membrane filtration-automated scanning electron microscopy (MD-ME-SEM), and microwave digestion-membrane filtration-light microscopy (MD-MF-LM). <br><br>METHODS: Sixty samples were randomly divided into 3 groups for diatom test using three methods, and the sample preparation time, degree of digestion and recovery rate of diatoms were compared. <br><br>RESULTS: The sample preparation time was the shortest with MD-MF-LM and the longest with SD-CE-LM (P<0.05). MD-ME-SEM and MD-MF-LM allowed more thorough tissue digestion than SD-CE-LM. MD-ME-SEM resulted in the highest total recovery rate of diatom, followed by MD-MF-LM and then by SD-CE-LM (P<0.05); the recover rate of different diatom species was the highest with MD-ME-SEM, followed by MD-MF-LM and SD-CE-LM (P<0.05). <br><br>CONCLUSION: SD-CE-LM has a low recovery rate of diatoms especially for those with lengths shorter than 40 µm or densities less than 1/5. With a high recovery rate and accuracy in diatom test, MD-ME-SEM is suitable for diagnosis of suspected drowning cases. MD-MF-LM is highly efficient, sensitive and convenient for forensic diatom test.<p /> <p>Language: zh</p>",
language="zh",
issn="1673-4254",
doi="",
url="http://dx.doi.org/"
}