@article{ref1,
title="Phenotype and functions of B cells in patients with acute brain injuries",
journal="Molecular immunology",
year="2015",
author="Chenouard, Alexis and Chesneau, Mélanie and Braza, Faouzi and Dejoie, Thomas and Cinotti, Raphael and Roquilly, Antoine and Brouard, Sophie and Asehnoune, Karim",
volume="68",
number="2 Pt A",
pages="350-356",
abstract="BACKGROUND: Brain injuries (BI) induce a state of systemic immunosuppression, leading to a high risk of pneumonia. In this pilot study, we investigated the status of B cell compartment in BI patients. <br><br>METHODS: A prospective observational study was performed in 2 intensive care units in a university hospital. Blood samples were collected in 14 patients at day 1 and day 7 after acute BI. The phenotype and the ability of B cells to secrete IL-10 were compared to 11 healthy volunteers (HV). <br><br>RESULTS: Among the circulating lymphocytes, the frequency of B cells was significantly higher in BI patients compared to HV (p<0.001). B cells from BI patients displayed an activated profil on day 7 after BI, reflected by a significantly higher proportion of CD27(+) memory (p=0.01) and CD27(+) IgD(-) switched memory B cells (p=0.02), as well as a significantly higher blood level of IgA (p=0.001) and IgM (p<0.001) as compared to day 1. The frequency of IL-10 secreting B cells (IL-10(+) B cells) on day 1 and day 7 was significantly lower in BI patients compared to HV (p<0.05). Interestingly, we observed that all BI patients with high frequency of IL-10(+) B cells on day 1 displayed an episode of pneumonia, and had a longer duration of mechanical ventilation and ICU stay compared to BI patients with low proportion of IL-10(+) B cells. <br><br>CONCLUSION: This study provides an extensive description of the phenotype and function of B cells in BI patients. Our results suggest that IL-10(+) B cells could play a major role in immunosuppression after BI.<p /> <p>Language: en</p>",
language="en",
issn="0161-5890",
doi="10.1016/j.molimm.2015.09.001",
url="http://dx.doi.org/10.1016/j.molimm.2015.09.001"
}