@article{ref1,
title="Rapid identification of pesticides in human oral fluid for emergency management by thermal desorption electrospray ionization/mass spectrometry",
journal="Journal of Mass Spectrometry",
year="2016",
author="Chen, Bai-Hsiun and Shin, Shyi-Jang and Shiea, Jentaie and Lin, Shiang-Jiun and Chen, Peng-Yu and Su, Hung and Lee, Chi-Wei",
volume="51",
number="2",
pages="97-104",
abstract="Self-poisoning with pesticides accounts for approximately one-third of all suicides worldwide. To expedite rescue in the emergency department, it is essential to develop a point-of-care analytical method for rapid identification of ingested pesticides. In this study, five of the most common pesticides ingested by self-poisoning patients in Taiwan were analyzed from oral fluid samples. Pesticide-oral fluid mixtures were applied on a cotton swab and then transferred into methanol. A metallic probe was used to sample the methanol solution for subsequent thermal desorption-electrospray ionization mass spectrometry analysis. Altogether, pesticide sampling, transfer, desorption, ionization, and detection took less than 1 min. The reproducibility of this method (n = 6) was shown in the observed low-relative standard deviation (<7%) in the detection of pesticide in oral fluid. The detection limits of the pesticides in oral fluid obtained from four human subjects by thermal desorption-electrospray ionization mass spectrometry were between 1-10 ppb with relative standard deviation 10.7%. Moreover, in this study, linear responses of five pesticides in oral fluid with concentrations between 1 ppb-1 ppm (R2 between 0.9938 and 0.9988) were observed. As the whole analytical process is extremely short, this technique allows for early non-invasive point-of-care identification of pesticides in the oral fluid of self-poisoning patients in the emergency room, providing important toxicological information for decision-making during critical resuscitation. Copyright © 2016 John Wiley & Sons, Ltd.<p /> <p>Language: en</p>",
language="en",
issn="1076-5174",
doi="10.1002/jms.3719",
url="http://dx.doi.org/10.1002/jms.3719"
}