@article{ref1,
title="Freeze-and-thaw stability and long-term-stability of 84 synthetic cannabinoids in serum",
journal="Drug testing and analysis",
year="2016",
author="Hess, Cornelius and Krueger, Lynn and Unger, Michael and Madea, Burkhard",
volume="ePub",
number="ePub",
pages="ePub-ePub",
abstract="Information about stability of synthetic cannabinoids is important to give recommendations for storage conditions in cases in which use of synthetic cannabinoids is suspected. In this study freeze and thaw stability (3 cycles at -20 °C at 1.5 ng/ml) and long term stability at room temperature, 4 °C and -20 °C (until 150 days) were tested by a validated liquid chromatographic mass spectrometric method for 80 synthetic cannabinoids. <br><br>RESULTS demonstrated good freeze and thaw stability for most of the substances. For 5 F-ABICA (72.6% of peak area of time point zero), 5 F- 2 ADB PINACA 2 Isomer (68.7 %), 5Cl-AB-PINACA (84.2 %), AB FUBINACA 2-Isomer (33.5 %), 5 F-PCN (89.7 %), ADB-FUBINACA (78.0 %), EG 018 (88.9 %) and PX-1 (89.4 %) three freeze- and thaw cycles led to absolute peak areas < 90 % compared to 6 control samples. For 51 of 84 substances stability in serum could be demonstrated after 315 days of storage at -20 °C. For 82 of 84 substances stability in serum could be shown for at least 1 month at -20 °C. Long term stability at 4 °C or 20 °C showed worse results. Therefore it is our recommendation to store real serum samples at -20 °C until analysis. This article is protected by copyright. All rights reserved.<br><br>This article is protected by copyright. All rights reserved.<p /> <p>Language: en</p>",
language="en",
issn="1942-7603",
doi="10.1002/dta.2133",
url="http://dx.doi.org/10.1002/dta.2133"
}