@article{ref1,
title="Quantitative determination of cannabinoids in cannabis and cannabis products using ultra-high-performance supercritical fluid chromatography and diode array/mass spectrometric detection",
journal="Journal of forensic sciences",
year="2016",
author="Wang, Mei and Wang, Yan-Hong and Avula, Bharathi and Radwan, Mohamed M. and Wanas, Amira S. and Mehmedic, Zlatko and van Antwerp, John and ElSohly, Mahmoud A. and Khan, Ikhlas A.",
volume="62",
number="3",
pages="602-611",
abstract="Ultra-high-performance supercritical fluid chromatography (UHPSFC) is an efficient analytical technique and has not been fully employed for the analysis of cannabis. Here, a novel method was developed for the analysis of 30 cannabis plant extracts and preparations using UHPSFC/PDA-MS. Nine of the most abundant cannabinoids, viz. CBD, ∆(8) -THC, THCV, ∆(9) -THC, CBN, CBG, THCA-A, CBDA, and CBGA, were quantitatively determined (RSDs < 6.9%). Unlike GC methods, no derivatization or decarboxylation was required prior to UHPSFC analysis. The UHPSFC chromatographic separation of cannabinoids displayed an inverse elution order compared to UHPLC. Combining with PDA-MS, this orthogonality is valuable for discrimination of cannabinoids in complex matrices. The developed method was validated, and the quantification results were compared with a standard UHPLC method. The RSDs of these two methods were within ±13.0%. Finally, chemometric analysis including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to differentiate between cannabis samples.<br><br>© 2016 American Academy of Forensic Sciences.<p /> <p>Language: en</p>",
language="en",
issn="0022-1198",
doi="10.1111/1556-4029.13341",
url="http://dx.doi.org/10.1111/1556-4029.13341"
}