@article{ref1,
title="Biopanning of allergens from wasp sting patients",
journal="Bioscience reports",
year="2018",
author="Chai, Lin and Yang, Xianyi and Liu, Mei and Liu, Chunyan and Han, Limei and Guo, Hui and Li, Changsheng and Sun, Yuwen and Li, Xiaoyan and Xiao, Min and Fang, Zhicheng",
volume="38",
number="5",
pages="ePub-ePub",
abstract="OBJECTIVE: Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of this study was to systematically study the epitopes of wasp venom. <br><br>METHODS: Using a random 12-peptide phage library, we performed antibody-binding epitope panning on 10 serum samples from wasp sting victims at 3 h and 4 days after the sting. The panning epitopes were identified by high-throughput sequencing and matched with wasp venom proteins by BLAST. The panned antibody-binding epitopes were verified by ELISA. <br><br>RESULTS: A total of 35 specific potential wasp venom epitopes in 4 days were identified. Among them, 12 peptide epitopes were matched with nine wasp venom proteins, namely, vitellogenin precursor, hexamerin 70b precursor, venom carboxylesterase-6 precursor, MRJP5, major royal jelly protein 8 precursor, venom acid phosphatase Acph-1 precursor, phospholipase A2, venom serine protease 34 precursor, and major royal jelly protein 9 precursor. The changes in serum IgM antibodies induced by wasp venom were confirmed by ELISA based on the 12 peptide epitopes. <br><br>CONCLUSION: The nine wasp venom proteins are potential allergens, which should be excluded or modified in the potential biomedical applications of wasp venom.<br><br>©2018 The Author(s).<p /> <p>Language: en</p>",
language="en",
issn="0144-8463",
doi="10.1042/BSR20181113",
url="http://dx.doi.org/10.1042/BSR20181113"
}