@article{ref1,
title="Myth-busting the zone of injury concept: a prospective study on the vascular response to high-energy lower extremity trauma",
journal="Plastic and reconstructive surgery",
year="2023",
author="Cepas, Adas and Kiiski, Juha and Majava, Marja and Kholová, Ivana and Kaartinen, Ilkka",
volume="ePub",
number="ePub",
pages="ePub-ePub",
abstract="BACKGROUND: Although the zone of injury concept is widely accepted, no histologic studies of vessel wall changes causing the phenomena are reported. This prospective study investigated the vascular response to high-energy lower extremity trauma to evaluate the validity of the zone of injury concept. <br><br>METHODS: The histologic appearance of arterial and venous walls in the zone of injury was studied in 19 patients (median age 46 [interquartile range 29.5-62.5] years) who underwent osteosynthesis and free-flap reconstruction due to high-energy lower extremity open fracture. Vascular samples were harvested from the injured extremity and control samples were harvested from the free-flap donor site. Histologic and morphometric characteristics of the vessels were analyzed microscopically and using digital pathology QuPath software. <br><br>RESULTS: Vascular samples were harvested on post-injury days 1-11. Intimal thickness was >3 times greater in arteries harvested from the zone of injury than in control samples (P<0.01) and the intima/media ratio was 2-fold that in control samples (P=0.01). Arterial intimal fibrosis was more evident in vessels harvested from the zone of injury (P<0.01), but medial fibrosis and medial thickness did not differ significantly between groups. Venous intimal thickening (P<0.01) and the intima/media ratio (P=0.02) were superior in samples from the zone of injury. Fibrosis-related changes did not differ between groups (P=0.45). <br><br>CONCLUSIONS: These findings support the validity of the zone of injury concept by providing a novel histologic basis for this phenomenon. Intimal thickening and arterial intimal fibrosis are prominent histologic features of vessels affected by major lower extremity trauma.<p /> <p>Language: en</p>",
language="en",
issn="0032-1052",
doi="10.1097/PRS.0000000000010980",
url="http://dx.doi.org/10.1097/PRS.0000000000010980"
}