@article{ref1,
title="New autophagy-modulating lanostane-type triterpenoids from a hallucinogenic poisonous mushroom Gymnopilus orientispectabilis",
journal="Archives of pharmacal research",
year="2024",
author="Lee, Seulah and Jang, Mina and Ryoo, Rhim and Roh, Jongtae and Ko, Sung-Kyun and Kim, Ki Hyun",
volume="ePub",
number="ePub",
pages="ePub-ePub",
abstract="Gymnopilus orientispectabilis, also known as &quot;big laughter mushroom,&quot; is a hallucinogenic poisonous mushroom that causes excessive laughter upon ingestion. From the fruiting bodies of G. orientispectabilis, eight lanostane-type triterpenoids (1-8), including seven novel compounds: gymnojunols A-G (2-8), were isolated. The chemical structures of these new compounds (2-8) were determined by analyzing their 1D and 2D NMR spectra and HR-EISMS, and their absolute configurations were unambiguously assigned by quantum chemical ECD calculations and a computational method coupled with a statistical procedure (DP4+). Upon evaluating autophagic activity, compounds 2, 6, and 7 increased LC3B-II levels in HeLa cells to a similar extent as bafilomycin, an autophagy inhibitor. In contrast, compound 8 decreased the levels of both LC3B-I and LC3B-II, and a similar effect was observed following treatment with rapamycin, an autophagy inducer. Our findings provide experimental evidence for new potential autophagy modulators in the hallucinogenic poisonous mushroom G. orientispectabilis.<p /> <p>Language: en</p>",
language="en",
issn="0253-6269",
doi="10.1007/s12272-024-01486-1",
url="http://dx.doi.org/10.1007/s12272-024-01486-1"
}