@article{ref1,
title="Imidazoline receptor proteins are decreased in the hippocampus of individuals with major depression",
journal="Biological psychiatry",
year="2000",
author="Piletz, J. E. and Zhu, H. and Ordway, G. and Stockmeier, C. and Dilly, G. and Reis, D. and Halaris, A.",
volume="48",
number="9",
pages="910-919",
abstract="BACKGROUND: A downregulation of I(2)-imidazoline binding sites has been reported in frontal cortices of depressed suicide victims, according to I(2)-radioligand binding and confirmed by Western blotting. We now report Western blots of imidazoline receptor proteins in hippocampi of subjects with and without depression at the time of death. <br><br>METHODS: Postmortem diagnoses were obtained from 17 cases of Axis I major depressive disorder and 17 cases without Axis I psychopathology. No psychotropic compounds were found in body fluids. Hippocampi were removed, sectioned, and assessed histologically. Throughout the analysis, each major depressive disorder sample was paired with a sample from a psychiatrically healthy subject based on equivalent life spans and postmortem delays. The antiserum was identical to that used in previous studies that reported a downregulation of cortical 29/30-kd imidazoline receptor-binding proteins in depression. <br><br>RESULTS: A triad of imidazoline receptor-binding protein bands (40-50 kd) was detected in the human hippocampus. Subjects with major depressive disorder had significantly less intensity in each imidazoline receptor-binding proteins band compared with control subjects (p =. 01 for overall bands). <br><br>CONCLUSIONS: The present results can be aligned with previous reports of downregulation of I(2)-radioligand binding sites in both cortices and platelets of depressed patients.<p /><p>Language: en</p>",
language="en",
issn="0006-3223",
doi="10.1016/s0006-3223(00)00892-1",
url="http://dx.doi.org/10.1016/s0006-3223(00)00892-1"
}