@article{ref1,
title="A novel in-vitro model of human aortic valve mineralization",
journal="Journal of heart valve disease",
year="2014",
author="Goncerz, G. and Tomaszewski, K.A. and Pasternak, A. and Głowacki, R. and Wróbel, A. and Rokita, E. and Podolec, P.",
volume="23",
number="5",
pages="545-549",
abstract="BACKGROUND AND AIM OF THE STUDY: The study aim was to develop an in-vitro model of human aortic valve homograft (AVH) mineralization, as a basis for further research on the subject of calcific aortic valve disease (CAVD).; METHODS: Human aortic valves were excised during autopsy (12-24 h post-mortem) from donors who had died due to accident or suicide. The mean (±SD) donor age was 32.5 ± 11.4 years. Under sterile conditions each aortic cusp (three cusps per valve) was cut in half to provide a total of 240 tissue samples. AVH mineralization was assessed in solutions with different Ca and P concentrations and CaxPO4 ionic product values. The impact of time on aortic valve mineralization rate was assessed using energy-dispersive X-ray fluorescence (ED-XRF).; RESULTS: The model showed that human AVH mineralization can be best observed in a solution with a CaxPO4 ionic product of 2.2 mmol2/l2. The optimum incubation time for observing AVH mineralization was 21 days.; CONCLUSION: A novel in-vitro AVH mineralization model was developed for use in future studies.<p /><p>Language: en</p>",
language="en",
issn="0966-8519",
doi="",
url="http://dx.doi.org/"
}