@article{ref1,
title="A double suicide gene system driven by KDR promoter selectively kills human colon adneocarcinoma SW480 cells",
journal="Nan Fang Yi Ke Da Xue Xue Bao",
year="2010",
author="Wang, Zhao-yang and Huang, Zong-hai and Li, Qiang and Yao, Xiao-jun and Yu, Jin-long and Li, Zhou",
volume="",
number="12",
pages="224-227",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To study the selective killing effect of adenovirus (Ad)-mediated double suicide gene system driven by the KDR promoter (KDR-CDglyTK) on human colon adneocarcinoma SW480 cells.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;KDR-expressing SW480 cells and LS174T cells that did not express KDR were infected by KDR-CDglyTK, and the infection efficiency and the expression of CDglyTK in the cells were detected by RT-PCR. The infected cells were treated with the prodrugs 5-FC and GCV at different concentrations, and the cell-killing effects and bystander effects were evaluated by MTT method. DNA content and the cell cycle changes in SW480 cells were detected by flow cytometry.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;The expression of green fluorescent protein (GFP) was observed in 95% of the infected SW480 and LS174T cells with a multiplicity of infection (MOI) of 100. RT- PCR demonstrated that the product of CD/TK gene existed in SW480 cells infected by Ad- KDR- CD/TK, but not in infected LS174 cells. The infected SW480 cells exhibited high sensitivity to the prodrugs, but the infected LS174T cells did not (P&lt;0.01). Bystander effects of the double suicide gene system were observed in the coculture of the infected and non-infected SW480 cells. At the MOI of 100, treatment of the infected cells with the prodrugs resulted in increased cell percentage in G(0)-G(1) phase and decreased percentage in S phase and the prodrug-treated cells showed an apoptotic peak in flow cytometry.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;CDglyTK fusion gene system driven by the KDR promoter selectively kills and induces the apoptosis of the KDR-CDglyTK SW480 cells.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1673-4254",
doi="",
url="http://dx.doi.org/"
}