@article{ref1,
title="A double suicide gene system driven by KDR promoter selectively kills human hepatic carcinoma cells and human umbilical vein endothelial cells",
journal="Nan Fang Yi Ke Da Xue Xue Bao",
year="2009",
author="Yang, Liu-cheng and Huang, Zong-hai and Kong, Heng and Li, Qiang and Chen, Fei and Yu, Jin-long and Li, Zhou",
volume="",
number="12",
pages="1-4",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To study the selective killing effects of adenovirus (Ad)-mediated double suicide gene system driven by KDR promoter (KDR-CdglyTK) on the human hepatic carcinoma cells and human umbilical vein endothelial cells (HUVECs).&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;KDR-expressing BEL-7402 and HUVECs and HepG2 cells that did not express KDR were infected by KDR-CdglyTK, and the infection efficiency and the expression of CdglyTK in the cells was detected by RT-PCR. The infected cells were treated with the the prodrugs 5-FC and GCV at different concentrations, and the cell-killing effects and bystander effects were evaluated by MTT method.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;At the multiplicity of infection (MOI) of 100, the recombinant AdKDR-CDglyTK showed similar infection efficiency in the 3 cell lines. RT-PCR demonstrated CDglyTK expression in the recombinant adenovirus and the 3 infected cell lines. BEL-7402 and HUVECs infected by the KDR-CdglyTK, but not the HepG2 cells, were highly sensitive to the prodrugs (P&lt;0.001). Bystander effects of the double suicide gene system were observed in the coculture of the infected and non-infected BEL-7402 and HUVECs.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;The double suicide gene system driven by KDR promoter has specific killing effect on KDR-expressing hepatocellular carcinoma cells and HUVECs.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1673-4254",
doi="",
url="http://dx.doi.org/"
}