@article{ref1,
title="Cell-killing effects of adenovirus-mediated transfer of CD :: UPP gene directed by mdr1 promoter on Taxol-resistant ovarian cancer cells",
journal="Tumor",
year="2007",
author="Lu, Shi",
volume="",
number="12",
pages="515-517",
abstract="OBJECTIVE: To evaluate the specific killing effects of the adenovirus-mediated transfer of cytosine deaminase gene and uracil phosphoribosyltransferase gene (CD::UPP) directed by the mdrl promoter combined with 5-fluorocytosine (5-FC) on Taxol-resistant ovarian cancer cells in vitro and in vivo. <br><br>METHODS: The adenovirus vector carrying the mdrl-CD :: UPP gene was amplified, purified, and transferred into Taxol-resistant A2780/Taxol ovarian cancer cells and A2780 cells. The expression of mdrl and CD :: UPP was detected by RT-PCR. After addition of 5-FC, the cell proliferation was assessed by MTT assay. The female BALB/c nude mice bearing tumor xenografts were injected adenovirus vector containing mdrl-CD :: UPP gene and then administered 5-FC. The growth of transplanted tumors was observed. <br><br>RESULTS: Mdrl and CD :: UPP gene were stably expressed in A2780/Taxol cells. The growth speed of A2780/Taxol cells was significantly slower than A2780 cells after transfection. Transfection of CD :: UPP gene combined with administration of 5-FC induced bystander effect of A2780/ Taxol cells on neighbor cells. The tumor growth in test group was significantly inhibited compared with control group (569.10 ± 187.93) mm3 vs (2 111.98 ± 230.82) mm3, P < 0.01. <br><br>CONCLUSION: The mdrl promoter in an adenovirus vector regulates the specific expression of CD :: UPP in A2780/Taxol cells. This system specifically kills Taxol-resistant ovarian cancer cells.<p /><p>Language: zh</p>",
language="zh",
issn="1000-7431",
doi="",
url="http://dx.doi.org/"
}