@article{ref1,
title="Construction of nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49",
journal="Lin chuang er bi yan hou tou jing wai ke za zhi",
year="2014",
author="Qing, Jing and Zhao, Suping and Jiang, Weihong and Zhang, Hua",
volume="",
number="24",
pages="178-181",
abstract="OBJECTIVE@#To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49.@*METHOD@#The plasmids of pcDNA3.1 (-)E6. BARF1p. CD/UPRT. UL49 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UL49 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE-2 cells.@*RESULT@#Suicide genes were expressed stably in CNE-2 cells.@*CONCLUSION@#We constructed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamine.<p /><p>Language: zh</p>",
language="zh",
issn="1001-1781",
doi="",
url="http://dx.doi.org/"
}