@article{ref1,
title="Construction of serine protease gene mutant and study on pathogenicity of serine protease gene of Entero-coccus faecalis",
journal="Chinese Journal of Infectious Diseases",
year="2000",
author="Wu, Li-Xian and Huang, Wen and Xiang, Sun and Xiao, Ping",
volume="",
number="12",
pages="-",
abstract="OBJECTIVE To construct the serine protease gene(sprE)mutant and to study the pathogenicity of sprE gene of Enterococcus faecalis.<br><br>METHODS Recombinant suicide vector pCQ001 of Enterococcus faecalis with pTX4577,was constructed.Then,created isogenic sprE-deficient mu- tant(*sprE)by allelic replacement was constructed.Moreover,the growth ability and the virulence of the mutant were compared with those of the wide type in vitro and in vivo respectively.A mouse peritonitis model and a rabbit endocarditis model were utilized in the study.<br><br>RESULTS The *sprE was selected by kanamycin and identified by polymerase chain reaction(PCR),pulsed field gel electropho- resis(PFGE)and Southern blot.The evidences showed that the sprE gene had a major role in helping bacteria to resist the elevated temperature and oxidative stress.The virulence of mutant decreased af- ter sprE gene was knocked out.<br><br>CONCLUSIONS The *sprE of Enterococcus faecalis is constructed suc- cessfully,sprE gene is important in the pathogenesis of Enterococcus faecalis,which probably is a major virulence factor of Enterococcus faecali.<p /><p>Language: zh</p>",
language="zh",
issn="1000-6680",
doi="",
url="http://dx.doi.org/"
}