@article{ref1,
title="Experimental study of tissue-specific suicide gene therapy for lung adenocarcinoma",
journal="Chinese Journal of Lung Cancer",
year="2004",
author="Zhao, Lijun and Li, Qiang and Bai, Chong and Li, Yuguang and Cao, Guangwen and Qi, Zhongtian",
volume="",
number="12",
pages="290-293",
abstract="&lt;p&gt;&lt;b&gt;BACKGROUND&lt;/b&gt;To study the effect and safety of tissue specific gene therapy of suicide gene for lung adenocarcinoma.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;Retroviral vector of G1CEACDNa contained a carcinoembryonic antigen (CEA) promoter regulated cytosine deaminase expression cassete (CEA/CD). By means of infection of virus, tissue specific expressing vectors and non-specific expressing vectors were transfected into A549 cell, which was CEA-producing lung adenocarcinoma cell line and then xenografted in nude mice, and the anti-tumor effect was evaluated. Then the retrovirus was injected directly into the tumor mass on nude mice, and the sensitivity of the xenograft to G1CEACDNa/5-fluorocytosine (5-FC) and the side effects were observed.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;(1) After transfected and untransfected A549 cells were implanted into nude mice, there was no difference in tumor formation among all the groups. (2) After 5-FC administration, the tumor transfected with tissue-specific gene displayed a higher sensitivity to the drug than those treated with non-specific in vitro gene-therapy. (3) The tumor-bearing nude mice were randomized in a blind manner based on comparable size to receive the supernatant of recombinant retrovirus G1CEACDNa followed by 5-FC, and significant growth suppression could be observed. (4) Comparing to the group with injection of 5-fluorouracil (5-FU) alone, tissue-specific suicide gene therapy showed lower suppression to bone marrow.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSIONS&lt;/b&gt;The results suggest that tissue-specific suicide gene therapy may play an important role in individual treatment of lung cancer.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1009-3419",
doi="10.3779/j.issn.1009-3419.2004.04.05",
url="http://dx.doi.org/10.3779/j.issn.1009-3419.2004.04.05"
}