@article{ref1,
title="Selective cytotoxic effect of lentivirus-mediated double suicide gene transfer on human gastric adneocarcinoma cells",
journal="Nan Fang Yi Ke Da Xue Xue Bao",
year="2010",
author="Kong, Heng and Huang, Zong-hai and Chen, Hai-jin and Li, Qiang and Tao, Lin-yu and Qi, Ke",
volume="",
number="12",
pages="47-50",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To study the selective cytotoxic effect of lentivirus-mediated double suicide gene (CD/TK) against human gastric carcinoma cells SGC-7901 in vitro.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;SGC-7901 cells were infected with FGW-KDRP-CD/TK vector and the infection efficiency was observed under a fluorescence microscope. The morphological changes of the infected cells were observed by Giemsa staining. Flow cytometry (FCM) was employed for cell cycle analysis, and the expression of CD/TK was detected by RT-PCR. The infected cells were then treated with the prodrugs ganciclovir (GCV) and/or 5-fluorocytosine (5-FC) at different concentrations, and the cytotoxic effects were evaluated using MTT method.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;The infection efficiency of the lentiviral vector in SGC-7901 cells increased with the titer of the virus, which produced no significant effect on the cancer cell morphology in vitro or on the percentages of G0-G1, G2-M and S phase cells (P&gt;0.05). RT-PCR demonstrated the expression of CD/TK gene in SGC-7901 cells infected by FGW-KDRP-CD/TK. The infected cells were highly sensitive to the prodrugs with a dose-dependent cytotoxic effect within a specific concentration range of the drugs, whereas the non-infected cells were not sensitive to the prodrugs. Combined use of the two prodrugs produced an obviously stronger inhibitory effect than either of the them (P&lt;0.05). When combined, GCV and 5-FC at the concentration of 0.1+40, 1+80, 10+160, and 100+320 mg/L demonstrated a synergetic effect with a CDI&lt;1.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;Lentivirus-mediated CD/TK fusion gene system can selectively kill gastric cancer cells, and the two prodrugs show a synergistic cytotoxic effect.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1673-4254",
doi="",
url="http://dx.doi.org/"
}