@article{ref1,
title="Study on AdCD-UPRT suicide gene mediated on lung cancer A_(549) in vitro",
journal="Journal of Chongqing Medical University",
year="2007",
author="Xu, Dan",
volume="",
number="12",
pages="-",
abstract="OBJECTIVE:To study combination AdCD-UPRT suicide gene mediated on lung cancer A549 in vitro. <br><br>METHODS:The recombinant AdCD-UPRT adenovirus was packaged and generated,and the title of virus was measured. The efficiency of transfecting target cells was detected,the toxicity of virus was measured,and the expression of CD-UPRT gene in A549 cells was assayed. The cytotoxicity and the bystander effect on A549 cells were measured by MTT assays respectively. The apoptosis was analyzed by AO-EB fluorescent staining,transmission electron microscope and flow cytometry. <br><br>RESULTS:The combination AdCD-UPRT had obviously growth inhibition effect on A549 cell and bystander effect. The cells were blocked in S phase and induced into apoptosis. <br><br>CONCLUSION:The combination AdCD-UPRT suicide gene had cytotoxicity on lung cancer A549 in vitro.<p /><p>Language: zh</p>",
language="zh",
issn="0253-3626",
doi="",
url="http://dx.doi.org/"
}