@article{ref1,
title="Study on the in vivo killing activity of YCD/5-FC gene therapy system on K562B cells",
journal="Chinese Journal of Hematology",
year="2002",
author="Zhang, Yusheng and Wang, Jianmin and Zhou, Hong and Zhai, Yongping",
volume="",
number="12",
pages="173-175",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To elucidate the killing activity of yeast cytosine deaminase/5-fluorocytosine (YCD/5-FC) gene therapy system on gene-transferred tumorigenic cell line K562B in vivo.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHOD&lt;/b&gt;K562B cell was infected with high titer virus and a gene transferred cell clone, YCD-K562B, was selected. Twelve male SCID mice of 4 week old were divided into 2 groups at random and both YCD-K562B and K562B cells were implanted to each mice. 5-FC or saline was given i. p for 10 days after tumor developed, and relative tumor volume was measured every 3 days. At the end of experiment, animals were sacrificed and the specimens were processed for histopathological examination.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;At the end of experiment (21 days after tumor cell implantation), the relative tumor volume of the 4 groups were: YCD-K562B + 5-FC 2.922 +/- 0.581, YCD-K562B + saline 24.434 +/- 4.790, K562B + 5-FC 22.701 +/- 2.350 and K562B + saline 24.460 +/- 1.670; t-test analysis showed that 5-FC could kill cells (YCD-K562B) in vivo (P = 0.0001), but had no effect on the growth of gene-untransferred cells (K562B) (P = 0.096). In YCD-K562B + 5-FC group, relative tumor volume reduced in 3 approximately 6 days after treatment (the minimum was 0.681). Necrosis around artery could be found in the tumor of YCD-K562B + 5-FC group.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;YCD/5-FC suicide gene therapy system has a significant in vivo killing activity to gene-transferred tumorigenic YCD-K562B cell.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="0253-2727",
doi="",
url="http://dx.doi.org/"
}