@article{ref1,
title="Targeted killing of colorectal tumor cells by lentiviral constructs containing CD/TK suicide genes and KDR promoter",
journal="Nan Fang Yi Ke Da Xue Xue Bao",
year="2007",
author="Chen, Hai-jin and Huang, Zong-hai and Wu, Ai-guo and Yu, Jin-long and Su, Guo-qiang",
volume="",
number="12",
pages="624-627",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To investigate the selective killing of colorectal tumor cells by lentivirus-mediated double suicide gene under the regulation of KDR promoter.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;293T packaging cells were transfected with the plasmid FGW-KDRP-CD/TK to obtain the infectious viruses. KDR-expressing LoVo cells and LS174T cells that did not produce KDR were transfected with the recombinant virus, and the transfection efficiency was evaluated by the fluorecence microscope. RT-PCR was employed to examine the expression of CDglyTK. After treatment of the cells with 5-FC and GCV, the killing effects on the two cell lines were evaluated.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;The recombinant construct showed similar infection rate of the two cell lines. RT-PCR demonstrated that CDglyTK gene was expressed only in LoVo cells infected with FGW-KDRP-CD/TK but not in LS147T cells, and the sensitivity of the two cell lines to the prodrugs was significantly different (P&lt;0.001). The killing effect of the double suicide gene was much stronger than that of single suicide gene administered (P&lt;0.001).&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;The double suicide gene driven by KDR promoter has specific killing effect on the KDR-expressing colorectal tumor cells.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1673-4254",
doi="",
url="http://dx.doi.org/"
}