@article{ref1,
title="The effects of HSV-tk suicide gene and wild-type p53 gene on pleomorphic adenoma cells of salivary gland",
journal="Hua xi kou qiang yi xue za zhi",
year="2005",
author="Wang, Xu and Wang, Jie and Dong, Fu-sheng and Dong, Yu-ying and Hou, Ya-li",
volume="",
number="6",
pages="65-68",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To study the therapeutic effects of combined gene therapy of wild type p53 (wt-p53) and herpes simplex virus thymidine kinase (HSV-tk) gene on pleomorphic adenoma cells of salivary gland.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;Wild type p53 and HSV-tk gene were transfected into human pleomorphic adenoma cells of salivary gland by using recombinant adenovirus vector. The efficiency of transfection was checked and gene was expressed by RT-PCR methods. The cell inhibition after transfected was verified by light microscope and MTT.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;The proliferation of the pleomorphic adenoma cells transfected wt-p53 and HSV-tk gene was inhibited and the cell survival rate decreased to 54% and 38% respectively in 5 days. However, when wt-p53 gene combined with HSV-tk/GCV system, the killing effects was significantly stronger (P &lt; 0.05) and the cell survival rate decreased to 20%.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;Combining p53 gene with HSV-tk/GCV system for gene therapy in pleomorphic adenoma cells of salivary gland is a valuable method.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1000-1182",
doi="",
url="http://dx.doi.org/"
}