@article{ref1,
title="The Death Mechanism of LoVo Cells Transfected with Cytosine Deaminase Suicide Gene",
journal="Journal of Chinese physician",
year="2000",
author="Wang, Xiaojun and Lai, Danian and Ma, Qingjiu",
volume="",
number="12",
pages="-",
abstract="OBJECTIVE To study the death mechanism of LoVo cells transfected with retroviral vector carrying cytosine deaminase (CD) suicide gene(G1CEACDNa). <br><br>METHODS Plasmid G1CEACDNa was transferred into the LoVo cells using liposomes method. RT-PCR was performed to detect CD mRNA expression using the total RNA extracted with TRIzol reagent. After exposed to 5-FC (1mmol?L -1 ), the cell growth inhibition rate and the &quot;bystander effect&quot; were determined by MTT, the cell ultramicroscopic structure was observed by electron microscope, and cell apoptosis was detected by flow cytometry. <br><br>RESULTS RT-PCR detection showed that there was 1.5kb band of CD product. The growth of the trasfected LoVo cells started to be inhibited after exposed 5-FC for 48h, and the inhibition rates at 72h,96h and 120h were 30%,50% and 80%, respetively.Electron microscope examination showed that there were apoptotic body. Meanwhile,a few cells showed necrosis alteration.Flow cytometry analysis showed that a few cells appeared apoptosis after exposed to 5-FC for 48h, the apoptotic rate and the necrosis rate at 72h and 96h were 23.8%, 35.1% and 20.4%, 26.0%, respectively. <br><br>CONCLUSION The death mechanism of LoVo cells transfected with plasmid G1CEACDNa followed 5-FC treatment was involved in both necrosis and apoptosis. Apoptosis possibly was a mechanism of the bystander effect.<p /><p>Language: zh</p>",
language="zh",
issn="1008-1372",
doi="",
url="http://dx.doi.org/"
}