@article{ref1,
title="The therapeutic mechanism of adenovirus mediated CD/5-FC system in the treatment of oral squamous carcinoma cells",
journal="Journal of Practical Stomatology",
year="2000",
author="Wang, Anxun and Huang, Hongzhang",
volume="",
number="6",
pages="-",
abstract="OBJECTIVE: To study the therapeutic mechanism of adenovirus mediated cytosine deaminase /5-fluorocytosine (AdCMVCD/ 5-FC) suicide gene system in the treatment of oral squamous carcinoma cells in vitro. <br><br>METHODS: 3H-thymidine ( 3H-TdR) incorporation assay, flow cytometry (FCM), transmission electron microscope and TUNEL (TdT-mediated dUTP Nick End Labeling)assay were used to detected the changes of Tca8113 cells after the treatment with AdCMVCD/5-FC system. <br><br>RESULTS: After treatment with CD/5-FC system, 3H-TdR incorporation rate of the cells decreased and significantly decreased between different MOI (multiple of infection) at 5-FC 10 -3 mol/L (P0.05); many apoptotic cells were found under transmission electron microscope and the positive rate of apoptotic cells increased from (4.40?0.87)% (before treatment) to (15.80?1.55)% (P<p /><p>Language: zh</p>",
language="zh",
issn="1001-3733",
doi="",
url="http://dx.doi.org/"
}