@article{ref1,
title="Toxic effects of CD-TK double suicidal gene system against prostate carcinoma cells",
journal="Nan Fang Yi Ke Da Xue Xue Bao",
year="2007",
author="Zhu, Wen-hui and Tan, Wan-long and Huang, He and Shi, Xiang-hua and Xie, Yi",
volume="",
number="12",
pages="479-481",
abstract="&lt;p&gt;&lt;b&gt;OBJECTIVE&lt;/b&gt;To evaluate the toxic effects of the CD-TK fusion gene systems against prostate carcinoma cell line RM-1 for assessing the value of suicidal gene therapy for prostate carcinoma.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS&lt;/b&gt;CD-TK fusion gene and green fluorescent protein (GFP) gene were transfected into RM-1 cells through adenovirus vectors. RT-PCR was used to demonstrate successful transfection and transcription of the suicidal genes. The toxic effects of 5-FC and GCV used alone or in combination on the transfected cells were observed by MTT assay, with the non-transfected RM-1 cells serving as control.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS&lt;/b&gt;Cytotoxic activity of CD/5-FC and TK/GCV systems against RM-1 cells was observed, and combined treatment with the two drugs resulted in significantly lowered survival of CD-TK-expressing cells (P&lt;0.05). After exposure to 5-FC and GCV for 72 h, the survival rate of the transfected cells decreased to 71.56% and 47.27%, respectively, and their combined use resulted in a survival rate as low as 18.46%.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSION&lt;/b&gt;CD-TK fusion double suicidal gene system can produce significantly stronger toxic effect against RM-1 cells in vitro than either of suicidal genes.&lt;/p&gt;<p /><p>Language: zh</p>",
language="zh",
issn="1673-4254",
doi="",
url="http://dx.doi.org/"
}