@article{ref1,
title="Ether Oxidation by an Evolved Fungal Heme-Peroxygenase: Insights into Substrate Recognition and Reactivity",
journal="Journal of fungi (Basel)",
year="2021",
author="Mireles, Raul and Ramirez-Ramirez, Joaquin and Alcalde, Miguel and Ayala, Marcela",
volume="7",
number="8",
pages="e608-e608",
abstract="Ethers can be found in the environment as structural, active or even pollutant molecules, although their degradation is not efficient under environmental conditions. Fungal unspecific heme-peroxygenases (UPO were reported to degrade low-molecular-weight ethers through an H2O2-dependent oxidative cleavage mechanism. Here, we report the oxidation of a series of structurally related aromatic ethers, catalyzed by a laboratory-evolved UPO (PaDa-I) aimed at elucidating the factors influencing this unusual biochemical reaction. Although some of the studied ethers were substrates of the enzyme, they were not efficiently transformed and, as a consequence, secondary reactions (such as the dismutation of H2O2 through catalase-like activity and suicide enzyme inactivation) became significant, affecting the oxidation efficiency. The set of reactions that compete during UPO-catalyzed ether oxidation were identified and quantified, in order to find favorable conditions that promote ether oxidation over the secondary reactions.<p /><p>Language: en</p>",
language="en",
issn="2309-608X",
doi="10.3390/jof7080608",
url="http://dx.doi.org/10.3390/jof7080608"
}