@article{ref1,
title="Development and application of a radioimmunoassay to detect interleukin-1 in rat peripheral circulation",
journal="American journal of physiology",
year="1992",
author="Derijk, R. and Berkenbosch, F.",
volume="263",
number="6",
pages="E1092-1098",
abstract="We describe the development and application of a radioimmunoassay to detect circulating interleukin (IL)-1 beta concentrations in the rat. No IL-1 immunoreactivity above the detection limit of the assay (100 pg/ml) could be detected in plasma of control rats. In contrast, immunoreactive IL-1 was detected after intravenous administration of rat recombinant IL-1 beta (rrIL-1 beta) or bacterial lipopolysaccharide (LPS) to rats. The effect of LPS on plasma immunoreactive IL-1 concentrations was time and dose dependent. The immunoreactive IL-1 response to LPS was prevented by in vivo macrophage depletion induced by liposome-directed macrophage suicide technique. Gel filtration of plasma from LPS-treated rats revealed the presence of a high and a smaller molecular form of immunoreactive IL-1. The small molecular immunoreactive IL-1 peak coeluted with rrIL-1 beta and probably represents the 17-kDa form of IL-1 beta. In conclusion, our data support the hypothesis that IL-1 secreted by macrophages can act as a humoral signal molecule to induce the immunological, metabolic, and neuroendocrine changes in response to bacterial LPS.<p /><p>Language: en</p>",
language="en",
issn="0002-9513",
doi="10.1152/ajpendo.2006.263.6.E1092",
url="http://dx.doi.org/10.1152/ajpendo.2006.263.6.E1092"
}