@article{ref1,
title="Simultaneous determination of chloroquine and its three metabolites in human plasma, whole blood and urine by ion-pair high-performance liquid chromatography",
journal="Journal of chromatography",
year="1992",
author="Houze, P. and de Reynies, A. and Baud, F. J. and Benatar, M. F. and Pays, M.",
volume="574",
number="2",
pages="305-312",
abstract="A method was developed for the separation and measurement of chloroquine and three metabolites (desethylchloroquine, bisdesethylchloroquine and 4-amino-7-chloroquinoline) in biological samples by ion-pair high-performance liquid chromatography with UV detection. The method uses 2,3-diaminoaphthalene as an internal standard and provides a limit of detection between 1 and 2 ng/ml for chloroquine and its metabolites. The assay was linear in the range 12.5-250 ng/ml and the analytical recovery and reproducibility were sufficient. The assay was applied to the analysis of biological samples from a patient undergoing chloroquine chemoprophylaxis and a patient who had ingested chloroquine in a suicide attempt.<p /><p>Language: en</p>",
language="en",
issn="",
doi="10.1016/0378-4347(92)80044-q",
url="http://dx.doi.org/10.1016/0378-4347(92)80044-q"
}