@article{ref1,
title="Measurement of inhibin A: a modification to an enzyme-linked immunosorbent assay",
journal="Prenatal Diagnosis",
year="2001",
author="Thirunavukarasu, P. P. and Wallace, E. M.",
volume="21",
number="8",
pages="638-641",
abstract="Inhibin A is a useful prenatal marker of Down syndrome. Currently, the available enzyme-linked immunosorbent assays (ELISAs) for inhibin A are based upon the same paired monoclonal antibodies. In the present study we have confirmed for one of those ELISAs that short-term sample storage as whole blood leads to a significant decline in detectable inhibin A and that this is most likely due to erythrocyte catalase interference with a critical oxidation step in the assay. While this interference can be eliminated by heating the samples pre-assay, this process is labour intensive. In the present study we have demonstrated that the addition of 3-amino-1,2,4-triazole (AT), a catalase 'suicide' inhibitor, also prevents the decline of inhibin A levels in samples stored as whole blood. We suggest that the addition of AT to samples prior to assay is a simple modification to the inhibin A ELISA that affords optimum performance.<p /><p>Language: en</p>",
language="en",
issn="0197-3851",
doi="10.1002/pd.127",
url="http://dx.doi.org/10.1002/pd.127"
}