@article{ref1,
title="Determination of glyphosate, glyphosate metabolites, and glufosinate in human serum by gas chromatography-mass spectrometry",
journal="Journal of chromatography B: analytical technologies in the biomedical and life sciences",
year="2008",
author="Motojyuku, Megumi and Saito, Takeshi and Akieda, Kazuki and Otsuka, Hiroyuki and Yamamoto, Isotoshi and Inokuchi, Sadaki",
volume="875",
number="2",
pages="509-514",
abstract="This paper describes an assay for the determination of glyphosate (GLYP), glyphosate metabolites [(aminomethyl) phosphonic acid] (AMPA), and glufosinate (GLUF) in human serum. After protein precipitation using acetonitrile and solid-phase extraction, serum samples were derivatized and analyzed by gas chromatography-mass spectrometry (GC-MS). The assay was linear over a concentration range of 3-100.0 microg/ml for GLYP, AMPA, and GLUF. The overall recoveries for the three compounds were >73%. The intra- and inter-day variations were <15%. Precision and accuracy were 6.4-10.6% and 88.2-103.7%, respectively. The validated method was applied to quantify the GLYP and AMPA content in the serum of a GLYP-poisoned patient. In conclusion, the method was successfully applied for the determination of GLYP and its metabolite AMPA in serum obtained from patient of GLYP-poisoning.<p /><p>Language: en</p>",
language="en",
issn="1570-0232",
doi="10.1016/j.jchromb.2008.10.003",
url="http://dx.doi.org/10.1016/j.jchromb.2008.10.003"
}