@article{ref1,
title="Mapping the lipolytic proteome of adipose tissue using fluorescent suicide inhibitors",
journal="Methods in molecular biology",
year="2009",
author="Schicher, Maximilian and Kollroser, Manfred and Hermetter, Albin",
volume="579",
number="",
pages="497-511",
abstract="Lipases are responsible for the hydrolysis of acylglycerols and cholesteryl esters in animals, plants, and microorganisms. In this chapter we describe a tool for the concomitant analysis of lipases in complex proteomes. For this purpose, the target enzymes are selectively and covalently labelled with fluorescent suicide inhibitors. Stable lipid-protein complexes are formed that are separated by gel electrophoresis and identified by mass spectrometry.<p /><p>Language: en</p>",
language="en",
issn="1064-3745",
doi="10.1007/978-1-60761-322-0_25",
url="http://dx.doi.org/10.1007/978-1-60761-322-0_25"
}