@article{ref1,
title="Identifying 8-hydroxynaringenin as a suicide substrate of mushroom tyrosinase",
journal="Journal of Cosmetic Science",
year="2010",
author="Chang, Te-Sheng and Lin, Meng-Yi and Lin, Hsuan-Jung",
volume="61",
number="3",
pages="205-210",
abstract="A biotransformed metabolite of naringenin was isolated from the fermentation broth of Aspergillus oryzae, fed with naringenin, and identified as 8-hydroxynaringenin based on the mass and (1)H- and (13)C-NMR spectral data. The compound showed characteristics of both an irreversible inhibitor and a substrate of mushroom tyrosinase in preincubation and HPLC analysis. These results demonstrate that 8-hydroxynaringenin belongs to a suicide substrate of mushroom tyrosinase. The partition ratio between the compound's molecules in the formation of product and in the inactivation of the enzyme was determined to be 283 +/- 21. The present study's results, together with our previous findings, which proved that both 8-hydroxydaidzein and 8-hydroxygenistein are suicide substrates of mushroom tyrosinase, show that 7,8,4'-trihydroxyl functional groups on flavonoids' skeletons play important roles in producing suicide substrate properties toward mushroom tyrosinase.<p /><p>Language: en</p>",
language="en",
issn="1525-7886",
doi="",
url="http://dx.doi.org/"
}