%0 Journal Article
%T 2,3,7,8-Tetrachlorodibenzo-p-dioxin kills immature thymocytes by Ca2+-mediated endonuclease activation
%J Science
%D 1988
%A McConkey, D. J.
%A Hartzell, P.
%A Duddy, S. K.
%A Håkansson, H.
%A Orrenius, S.
%V 242
%N 4876
%P 256-259
%X Suspensions of thymocytes from young rats were incubated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which resulted in a sustained increase in cytosolic free Ca2+ concentration followed by DNA fragmentation and loss of cell viability. Both the Ca2+ increase and DNA fragmentation were prevented in cells treated with the inhibitor of protein synthesis, cycloheximide, and DNA fragmentation and cell killing were not detected when cells were incubated in a "Ca2+-free" medium or pretreated with high concentrations of the calcium probe, quin-2 tetraacetoxymethyl ester. These results indicate that TCDD can kill immature thymocytes by initiating a suicide process similar to that previously described for glucocorticoid hormones.<p /><p>Language: en</p>
%G en
%I American Association for the Advancement of Science
%@ 0036-8075
%U http://dx.doi.org/