TY  - JOUR
PY  - 1994//
TI  - Traumatic injury induces interleukin-6 production by human astrocytes
JO  - Brain research
A1  - Hariri, R. J.
A1  - Chang, V. A.
A1  - Barie, P. S.
A1  - Wang, R. S.
A1  - Sharif, S. F.
A1  - Ghajar, J. B.
SP  - 139
EP  - 142
VL  - 636
IS  - 1
N2  - The brain is being evaluated as a de novo source of cytokines. Because recent evidence indicates that interleukin-6 (IL-6) may influence blood-brain barrier function and vascular permeability, we have sought to determine whether mechanical injury can directly induce in situ cerebral IL-6 production. Adult human astrocyte cultures were subjected to mechanical injury by the in vitro method of fluid percussion barotrauma, developed in our laboratory. Serial supernatant samples were collected for 8 h and evaluated for IL-6 activity using a proliferation assay employing the dependent B cell hybridoma cell line, B9. At optimum injury, the IL-6 level became significantly (P < 0.0001, analysis of variance) elevated from baseline 2 h after trauma and continued to increase over the observation period. Our study shows that following mechanical injury human astrocytes produce IL-6, which may contribute to post-traumatic cerebrovascular dysfunction. Elucidating the precise role of intracerebral cytokines is essential to our understanding of the mechanism responsible for post-traumatic cerebrovascular dysfunction.<p /> <p>Language: en</p> 
LA  - en
SN  - 0006-8993
UR  - http://dx.doi.org/
ID  - ref1
ER  -