TY  - JOUR
PY  - 2010//
TI  - Cross reactivity between European hornet and yellow jacket venoms
JO  - European annals of allergy and clinical immunology
A1  - Severino, M. G.
A1  - Caruso, B.
A1  - Bonadonna, P.
A1  - Labardi, D.
A1  - Macchia, D.
A1  - Campi, P.
A1  - Passalacqua, G.
SP  - 141
EP  - 145
VL  - 42
IS  - 4
N2  - BACKGROUND: Cross-reactions between venoms may be responsible for multiple diagnostic positivities in hymenoptera allergy. There is limited data on the cross-reactivity between Vespula spp and Vespa crabro, which is an important cause of severe reactions in some parts of Europe. We studied by CAP-inhibition assays and immunoblotting the cross-reactivity between the two venoms. METHODS: Sera from patients with non discriminative skin/CAP positivity to both Vespula and Vespa crabro were collected for the analyses. Inhibition assays were carried out with a CAP method, incubating the sera separately with both venoms and subsequently measuring the specific IgE to venoms themselves. Immunoblotting was performed on sera with ambiguous results at the CAP-inhibition. RESULTS: Seventeen patients had a severe reaction after Vespa crabro sting and proved skin and CAP positive also to vespula. In 11/17 patients, Vespula venom completely inhibited IgE binding to VC venom, whereas VC venom inhibited binding to Vespula venom only partially (<75%). In 6 subjects the CAP-inhibition provided inconclusive results and their sera were analysed by immunoblotting. The SDS-PAGE identified hyaluronidase, phospholipase A1 and antigen 5 as the main proteins of the venoms. In 5 sera the levels of IgE against antigen 5 of Vespa crabro were higher than IgE against Vespula germanica, thus indicating a true sensitisation to crabro. CONCLUSION: In the case of multiple positivities to Vespa crabro and Vespula spp the CAP inhibition is helpful in detecting the cross-reactivities.<p />  <p>Language: en</p>
LA  - en
SN  - 1764-1489
UR  - http://dx.doi.org/
ID  - ref1
ER  -